人乳头瘤病毒在人大肠癌中检出

Detection of human papillomavirus in human colorectal cancer

用聚合酶链反应(Polymerase chain reaction,PCR)法对28例大肠癌(均为分化程度不同的腺癌)及18例相应癌旁组织中人乳头瘤病毒(human papillomavirus,HPV)16和18型DNA分别进行检测。在28例大肠癌组织中分别检测到3例(10.8％)呈HPV16DNA阳性和18例(64.5％)呈HPV18DNA阳性。而相应的18例癌旁组织中仅检测到3例(16.7％)呈HPV18DNA阳性,未检测到HPV16DNA。为了证实扩增产物的特异性,我们用限制性内切酶对PCR产物分别进行了酶切分析,结果与预期的完全相符。本文提示HPV感染可能是大肠癌的发病因素之一。

More than 60 types of human papillomaviruses ( HPV ) have been, reported. Among them, HPV types 16 and 18 are frequently found to be associated with carcinomas in lower urogenital tract especially the cervix uteri. Recently, HPVs have been detected in many other cancer tissues. However, whether colorectal ca-scinomas are associated with HPV infection is still in dispute. In this study we detected HPV16 and 18 DNA in colorectal carcinoma tissue ( n = 28 ) by polyme-rase chain reaction ( PCR ) , with the tissue outside the carcinomatous lesion as controls ( n = l8 ) . HPV18 DNA was shown to be 18/28 positive in the carcinoma tissue and 3/18 in the controls; HPV16 DMA was shown to be 3/28 positive in the carcinomas and none in the controls. In order to testify the specificity of the amplified fragments, we use specific restriction enzymes to cut the PCR products. The size of the resulting fragments conformed perfectly to that expected.Our study suggests that HPV may be one of the causative agents in te pathogenesis of colorectal carcinomas.