海水及氨水对九孔鲍卵催熟的研究

Studies on the relation between eggs of Haliotis diversicolor aquatilis and fertilization time

分别于水温 2 6℃、盐度 32的条件下和水温 2 4℃、盐度 32以及产卵后用 0 .0 0 2 %的氨水刺激卵子的条件下 ,利用光学显微镜观察了九孔鲍排出体外的成熟未受精卵的形态 ,并研究了形态与时间、受精与时间的关系。实验结果表明 ,在水温为 2 6℃、盐度为 32的条件下 ,卵子在排出后开始吸水膨胀 ,卵趋于卵膜中间。产卵 2 0 min后 ,卵子吸收了一定水分 ,卵才有受精能力 ,在排卵后 2 5～ 5 0min,在用有很强活力的精子的前提下 ,受精率达到最高值 ,而在排卵 2 h后 ,卵子开始失水 ,卵趋于卵膜一侧 ,卵的受精能力开始大幅度下降。在水温 2 4℃、盐度为 32及产卵后用 0 .0 0 2 %的氨水刺激卵子的条件下 ,6 min后 ,卵子吸收了一定水分 。

The paper deals with the relation between the fertilization rate and fertilization time,between the time and the morphology of natural eggs of Haliotis diversicolors aquatilis under the condition of combination of sea water temperature 26 ℃, salinity 32 and the condition of combination of water temperature 24 ℃, salinity 32 and the treatment of NH 3 by optical microscope. Under the condition of combination of water temperature 26 ℃ and salinity 32,the processions of egg variation that we observed were that eggs began to absorb water and expand after they were laid, and then tended to be in the middle of the egg membrane. After 20 minutes that they were laid, eggs absorbed certain water and had the capability of fertilization. After 25～50 minutes that they were laid, fertilization rate reached a maximum. However, after two hours that they were laid, eggs began to lose lots of water and tended to one side of egg membrane. At the same time, the fertilization capability of the eggs began to decrease sharply. Under the condition of combination of sea water temperature 24 ℃, salinity 32 and the treatment of NH 3, eggs began to absorb seawater and expand after 6 minutes when they were laid, and then tended to be ready for zygote.