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应用RAPD标记分析黑麦属的遗传多样性 被引量:6

Genetic diversity of Secale L. based on RAPD analysis
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摘要 采用随机扩增多态性DNA(RAPD)标记,对黑麦属(SecaleL)7个种共12份材料进行了遗传多样性检测。结果表明,被测材料间RAPD标记多态性较高。40个随机引物中,有25个引物(占62 5%)的扩增产物具有多态性。25个引物共扩增出167条带,其中89条带(占53 2%)具有多态性,每个引物可扩增出1~10条多态性带,平均3 6条。RAPD标记遗传距离(GD)变异范围为0 1382~0 4512,平均值为0 2712。聚类分析表明,在GD值0 3850水平上,12份材料可聚3类,S.africamum和S.sil vestre与其它材料间的遗传分化较大,分别单独聚为一类,其余10份材料则聚为一类。据此认为,RAPD标记可以作为黑麦属物种鉴定的指纹图谱。 Genetic diversity of 12 accessions of Secale L., distributed to 7 species,was analyzed based on random amplified polymorphic DNA (RAPD) markers.Fourty arbitrary primers (10mer) were used for the PCR amplification of genomic DNA, among which 62.5?% primers could produce polymorphic bands.A total of 167 bands were amplified from 25 primers,while 89 bands (about 53.2?%) were polymorphic. One to 10 polymorphic bands could be amplified from each primer,with an average of 3.6 bands.The mean genetic diversity(GD) value based on RAPD data among 12 accessions of Secale L.was 0.2712, ranging from 0.1382 to 0.4512.At the level of GD 0.385,cluster analysis showed all materials could be classified into 3 subclusters,in which S.africanum and S.silvestre were less related with others. These results suggested that RAPD markers could be used as one fingerprint in Secale.
出处 《西南农业学报》 CSCD 2002年第4期30-34,共5页 Southwest China Journal of Agricultural Sciences
基金 国家教育部重点项目(00225) 四川省教育厅预研项目(2000-CO2) 四川省科技厅农业生物技术专项基金项目
关键词 应用 RAPD标记 黑麦属 遗传多样性 遗传距离 聚类分析 Secale L. RAPD markers genetic distance genetic diversity cluster analysis
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