SSR分子标记的开发技术研究进展

Progress in the way to develop SSR molecular marker

综述简单重复序列标记(SSR)的特点及在作物遗传图谱构建、品种鉴定及分子标记辅助育种等方面的应用价值。重点描述开发SSR标记的两种方法的基本原理和主要步骤,一种是通过克隆酶切片段和大量测序寻找SSR标记的传统方法,一种是应用SAGE原理不需要克隆的STMP技术。介绍这两种方法在创建小麦或大豆SSR标记中的应用。它们都能有效地开发可扩增出特定位点SSR的PCR引物。

The characteristics of simple sequence repeats(SSR)and its importance in genetic mapping,variety identifying and molecular markerassistant breeding,etc,are discussed in this article.And two ways which can develop SSR molecular markers with their basic principles and main steps are also shown in this article.One is the traditional way which needs a great number of cloning of enzymecut DNA fragments and a great deal of sequencing,the another is a new way which can eliminate the cloning step and can reduce much more work of sequencing by the basic principle of serial analysis of gene expression(SAGE).The utility of these two ways in the development of bread wheat or soybean SSR is shown.The results showed that they were effective in the designing of PCR primers to amplify locusspecific SSR.