牛体外受精卵5～10细胞期单一卵裂球染色体诊断

Chromosomal diagnosis in each individual blastomere of 5-to 10-cell bovine embryos derived from in vitro fertilization

利用单一卵裂球的染色体诊断牛体外受精 5 -～ 1 0 -细胞期胚胎的正常性。牛体外受精后 5 -～ 1 0 -细胞期的胚胎用0 .5 %链霉蛋白酶处理分离单一卵裂球 ,然后用 1 0 0 ng/ml长春花碱处理 1 0 h,制作染色体标本。检查 3 3枚不同发育期胚胎及卵裂球 1 85个 ,有 43 .8%的可进行染色体分析 (81 /1 85 )。结果表明 ,正常 2倍体的发生率为 46.9% ,染色体异常的卵裂球中 ,单倍体的发生率为 5 0 .6% ,显著高于多倍体 (2 .5 % )的发生率 (P<0 .0 0 1 )。单倍体中含有 X-性染色体和含有 Y-性染色体的性比基本相同。

Chromosomal normality and sex were diagnosed in each blastomere of bovine embryos derived from in vitro fertilization(IVF). Bovine embryos developing to the 5 ～10 cell stage were separated into individual blastomere with 0 5% protease. After treatment with 100 ng/ml vinblastine sulfate for 10 h,they were prepared for chromosome samples. In total, 33 bovine embryos and 185 of the blastomeres were examined. 43 8% of which can be use for chromosome analysis (81/185). The result showed that the incidence rate of normal cliploid was 46 9% while that of haploid in the blastomere with abnormal chromosome was 50 6% and significantly higher (50 6%) than that polyploid embroyos(2 5%). The gender ratio of X chromosome and Y chromosome was almost same in the haploid. The occurrence of haploid may be caused by parthenogenesis of oocytes or by male pronucleus when the sperm penetrates into the immature ovum during fertilization.