活性氧及膜质过氧化与花生抗黄曲霉侵染的关系

Involvement of acitve oxygen generation and lipid peroxidation in susceptibility/resistance of peanut seed infected by Aspergillus flavus

以抗感花生品种的种子为材料,采用人工接种方法,研究种子受黄曲霉侵染前后活性氧和膜质过氧化的动态变化。结果表明,黄曲霉侵染可诱导花生种子发生膜质过氧化反应,抗感品种间存在明显的差异。抗性品种·产生速率、H2O2和MDA含量等比感病品种提高的速度快,发生量较大。但活性氧清除酶的脂氧合酶活性、O2CAT和SOD活性变化不明显,使抗病品种膜质过氧发生较早且程度较高,表皮细胞发生及时性的过敏性反应坏死,抵御黄曲霉的穿透,达到抗黄曲霉侵染的目的。

Active oxygen and lipid peroxidation levels in peanut seeds with different resistant to A.flavus invasion were analyzed after inoculation. The results showed that the lipoxygenase (LOX) activities, O2· generation rates, H2O2 level and MDA content in resistant cultivars were significantly increased at early stage after inoculation, while the SOD and CAT activities reminded unchanged. These results suggested that the accumulation of avtive oxygen species, the increasing of LOX activity and high lipid peroxidation levels in peanut seeds after inoculation might play an important role in resistance to A.flavus invasion.