摘要
将强启动子Ps插入载体7S中构建成插入载体7SP,将鸡马立克氏病病毒(MDV)糖蛋白B(gB)基因和禽流感病毒(AIV)血凝素H9A基因共同克隆到插入载体7SP中,获得重组转移质粒7SPGA,通过酶切鉴定获得Ps-gB与P7.5-HA同向的转移质粒,再将报告基因盒PL11-LacZ插入转移质粒7SPGA得7SPLGA,将质粒7SPLGA与禽痘病毒疫苗株共转染鸡成纤维细胞,通过蓝白斑筛选纯化得到重组病毒rFPV-Ps-gB-P7.5-HA,以间接免疫荧光法证实gB基因和HA基因同时得到表达。该研究为探索新型基因工程苗打下了良好的基础。
Recombinant fowlpox viruses (rFPVs) coexpressing Marek′s disease virus (MDV) glycoprotein B (gB) and Avian influenza (AIV) HA gene were constructed by using different promoters of Ps and P7.5, rFPVS were selected and purified by blue plaque expressing βgalactosidase. The expression of MDV gB gene and AIV HA gene were confirmed by indirect immunofluorescence assay.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
2002年第4期1-3,7,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家高新技术研究发展计划项目(863960332025)
