耐盐基因HAL1的克隆及植物表达载体的构建

CLONING OF HAL1 GENE FROM SACCHAROMYCES CEREVISIAE AND CONSTRUCTION OF ITS PLANT EXPRESSION VECTOR

对编码调节离子转运蛋白的基因HAL1进行克隆、序列测定,并对植物表达载体进行构建。基因测序分析表明:该基因含有885个核苷酸,与GenBank上公布的HAL1基因的核苷酸同源性为99.30%,氨基酸序列同源性为99.32%。利用Hind 和Xba 切点插入质粒pBY505的Actin启动子与PIN终止子之间,构建成适宜于直接转化法转化植物的表达载体pBHAL1。

Taking Saccharomyces cerevisae Y2 genomic DNA as template, HAL1 gene was amplificated with PCR. Moreover the PCR product of HAL1 was cloned into pGEMT easy vector. The gene was sequenced and the results showed that the HAL1 gene contains 885 nucleotides and 99.6% identity with published sequence. The HAL1 gene was constructed on the plant expression vector pBY505.