中国柞蚕DNA多态性的RAPD分析

RAPD Analysis of DNA Polymorphism of Antheraea pernyi

用随机扩增多态DNA(RAPD)标记技术对 4个代表性的柞蚕品种河 41、四青、青黄 1号、杏黄和 3个家蚕品种大造、C10 8、75 32的 2 8个个体进行了DNA多态性分析。结果表明 :柞蚕具有极为丰富的DNA多态性 ;不同品种的个体间 (种内 )的多态性为 80 7%,而同一品种个体间的多态性也达到 45 8%～ 49 4 %;同一品种个体间的遗传距离为 0 133～ 0 2 38,远大于家蚕的 0 0 0 8～ 0 0 81;不同品种个体间的遗传距离为 0 2 15～ 0 382 ,与家蚕相似。柞蚕的DNA多态性有 6 0 %来源于品种内的个体间 ,而来源于品种间的部分只占 40 %。UPGMA聚类时 ,柞蚕的各个体均能按品种聚在一起。

DNA Polymorphism in each 4 individuals of 4 Antheraea pernyi varieties (He41,Siqing,Qinghuang No.1,Xinghuang) and 3 Bombyx mori varieties (Dazao,C108,7532) was analyzed with Random Amplified Polymorphic DNA(RAPD) technique.The results showed that Antheraea pernyi has abundant DNA polymorphism.The DNA polymorphism of different individuals among different varieties is 80.7%,and that of different individuals within same variety reaches 45.8%～49.4%.The genetic distance (D) value of different individuals within same variety is 0.133～0.238,higher than Bombyx mori which is 0.008～0.081;The D value between different varieties is 0.215～0.382,same as Bombyx mori which is 0.206～0.356. 60% DNA polymorphism of Antheraea pernyi is derived from different individuals within same variety,and 40% from different varieties.