急性出血坏死性胰腺炎时胰腺微循环的病理形态学观察及NO在胰腺微循环障碍形成中的作用探讨

Pathomorphological observation of pancreatic microcirculation in acute hemorrhagic and necrotic pancreatitis and investigation of the role of NO in the development of pancreatic microcirculation disturbance

目的 :从急性出血坏死性胰腺炎 (AHNP)早期开始动态观察胰腺微循环的病理形态学变化 ,探讨NO在胰腺微循环障碍形成中的作用。材料与方法 :胰胆管内逆行注射 5 %的牛磺胆酸钠 ,建立急性出血坏死性胰腺炎模型 ,通过光镜及电镜观察胰腺微循环的病理形态学变化 ,用免疫组化SP法测定胰腺微血管内皮细胞内eNOS的表达情况。结果 :诱导AHNP后 5min就出现微血管损伤 ,随着疾病的发展损伤加重 ;从诱导AHNP后 10min开始 ,eNOS表达显著性增高 。

Objective: To observe the pathomorphological changes of pancreatic microcirculation in the early phase of acute hemorrhagic and necrotic pancreatitis. To investigate the role of NO in the development of the microcirculation disturbance. Material and method: AHNP was induced in rats(30, weight 300～350g) by intraductal administration of 5% sodium taurocholate. The pathologic morphologic changes of pancreatic blood micro vessel and acinus were observed under light microscope and electron microscope; The expression of eNOS in endothelium was examined through Immunohistochemistry. Results: The injuries were found in pancreatic micro vessel 5min after the inducement, accompanied by acinus coagulation necrosis. These changes were aggravated with the development of the disease. The expression of eNOS in endothelium of pancreatic micro vessel began to significantly increase 10min after the administration( p <0.05) and continue to increase with the development of the disease.