肥城桃组培苗诱导、基因转化及其增殖

Gene Transformation of in vitro Plantlets of Peach and Its Proliferation

红里、白里是肥城桃的两个优良品种,但不耐贮藏。为试图利用基因工程解决这一问题,先通过组培将其胚、胚乳、子叶的愈伤组织分别诱导再生植株和芽剥离出茎尖培育成苗。再将肥城桃反义PG基因,通过农杆菌介导转化组培苗,转化苗在含卡那霉素培养基上筛选,获得抗卡那霉素的转基因苗。用研制的增殖培养基培养4～7周,一个苗可生长出14～18分枝的苗,解决了转基因苗获得率低的问题,也满足了对转基因株系进行检测和从组培室到温棚到田间果园过渡栽培和芽接的需求,提高了繁育速率。

The Hongli and Baili,two selections of the famous peach variety Feicheng were used as materials in this experi-ment intending improving their storeability by gene transformation.Polygalacturonase has been known as an enzyme which de-polymerizes pectin in fruit cell wall;it expresses activity at the ripening stage of fruit.The aim of this experiment was to trans-forming the antisense polygalaturonase gene into the plantlets to gain a new variety with a better storage life.Experiment pro-cess included inducing regenerated plantlets from embryo,endosperm,cotyledon and tip of stem cultured,transgene and proliferation of transgenic plant.Now the transgenic plantlets have been obtained in this experiment.