含有绿色荧光蛋白基因的质粒载体的构建及其在水稻白叶枯细菌中的表达

Construction of the Plasmid Vector Carrying GFP Gene and the Expression of Xanthomonas Oryzae pv. Oryzae (Xoo) in Rice

本文用绿色荧光蛋白基因(greenfluorescentprotein)标记水稻白叶枯细菌,观察其在白叶枯细菌中的表达情况。光激发后,白叶枯细菌发出绿色荧光,表明gfp在白叶枯细菌中得到了高效表达。后续工作意在利用gfp所发出的绿色荧光,来追踪白叶枯细菌侵染水稻的路径,以及检测水稻在遭受白叶枯病害时的一些生理生态变化,进一步探讨水稻对白叶枯细菌的抗生机理,希望能够为水稻抗性品种的检测提供新的理论依据。文中重点介绍了对质粒pM2464的改造过程,经gfp标记后的水稻白叶枯细菌,在紫外或蓝光的激发下,发出绿色荧光,证明了用标记有gfp基因的白叶枯细菌来观察其侵染水稻过程的想法是可行的。

This paper introduced Xanthomonas oryzae pv. oryzae was marked with green fluorescent protein (gfp) gene and observation the expression of gfp. Excited with light, the pathogen emitted green fluorescence, showed gfp was expressed high effciently. In the followup work, by tracing the green fluorescence, can be known the infestation path of the pathogen in rice and the physiological changes of irce infracted by pathogen; so the disease resistance mechanism of rice versus pathogenic bacteria can be further probed into through the above work. In this paper the process of reconstruction plasmid pMP2464 was introduced particularly, the pathogen marked with gfp was excited by ultraviolet or blue light and emitted fluorescence, this all proved that the idea stated as previous is feasible.