濒危植物七子花DNA的提取及分析

Extraction and determination of DNA from Heptacodium miconioides

用不同的方法抽提濒危植物七子花嫩叶DNA ,利用紫外分光光度法对其质量进行鉴定 ,以紫外分光光度法及琼脂糖凝胶电泳 -溴化乙锭染色法对其含量进行双重测定 ,显示适合七子花DNA抽提的最佳方法是改进的SDS法 ,该法适合RAPD分析。用此法对七子花植株不同器官的DNA进行抽提 ,其含量分别是 :嫩叶 >枝芽 >老叶 >嫩茎 >老茎。

DNA was extracted from tender leaves of Heptacodium miconioides by using different methods. The quality was determined by UV spectrometers and the quantity was doubly determined by electrophoresis and spectrometers. The best methods for the DNA extraction of Heptacodium miconioides was improved SDS method,which was suitable for PCR analysis. DNA was extracted from different organs of Heptacodium miconioides using this method. It showed that the maximal DNA content was in tender leaves,and followed by older leaves,twig,older stem,side-bud.