应用正交设计建立青花菜植株的再生体系

Establishment of broccoli plant regeneration system with orthogonal design

通过L16(4 5)正交试验 ,研究最适合青花菜 2周龄下胚轴愈伤组织诱导和不定芽发生的植物生长调节物质的种类和浓度组合。结果发现在NAA、6 BA、TDZ和KT四种激素中 ,NAA对下胚轴愈伤组织发生指数、不定芽发生频率的影响作用最大 ,确定以MS +NAA 0 .2 5mg/L +6 BA 0 .2 5mg/L +TDZ 0 .0 1mg/L(琼脂 0 .8% ,蔗糖 3 % ,pH5 .8)作为单因子试验的培养基。NAA、6 BA和TDZ浓度的单因子试验结果表明最适合下胚轴的培养基配方为 :MS +NAA 0 .1mg/L +6 BA 1 .0mg/L +TDZ 0 .0 6mg/L(琼脂 0 .8% ,蔗糖 3 % ,pH5 .8)。

Different kinds and concentrations of 4 plant hormones were tested with orthogonal design-L 16(45)for their optimal effect on shoot regeneration and callus inducement in broccoli hypocotyls. The results showed that the effect of the four plant hormones to shoot regeneration frequency and callus formation index is in the order of NAA>TDZ>KT>6-BA and NAA>TDZ>KT>6-BA respectively, and the best medium for broccoli hypocotyl culture is MS+NAA 0.1 mg/L+6-BA 1.0 mg/L+TDZ 0.06 mg/L+agar 0.8% containing sugar 3%(pH5.8).