温度对六种外来杂草过氧化物酶同工酶谱的影响

Influences of temperature on peroxidase isozymograms of six exotic weeds

应用聚丙烯酰胺垂直板凝胶电泳 ,比较了加拿大一枝黄花 (Solidagocanadensis)、小飞蓬 (Conyzacanadensis)、野塘蒿 (Conyzabonarinsis)、钻形紫菀 (Astersublatus)、一年蓬 (Erigeronannuus)和马缨丹 (Lan tanacamara)等 6种外来杂草在 3 8、2 5、5°C处理 60h后的过氧化物酶同工酶谱差异 ,分析了酶谱差异与对温度适应的关系。结果表明 ,温度变化对一年蓬、小飞蓬、钻形紫菀过氧化物酶同工酶谱的影响相对较小 ,野塘蒿通过增加酶带、调整同工酶的组成来适应温度的变化 ,反映出这 4种杂草对温度变化具有较强的适应能力 ;加拿大一枝黄花表现出对高温的适应能力较弱 ,对低温适应性较强 ,马缨丹对温度变化表现出相反的适应特点。

By polyacyacrylamide gel electrophoresis,peroxidase isozymes of six exotic weeds including Solidago canadensis,Conyza canadensis,Conyza bonarinsis,Aster sublatus,Erigeron annuus and Lantana camara were surveyed for 60 h under 5 °C,25 °C and 38 °C. The variance of POD isozymograms of the six exotic weeds under different temperatures and their adaptation to temperature were analyzed. The results showed:(1)No obvious changes of POD isozymograms were found in Erigeron annuus,Conyza canadensis and Aster sublatus when temperature changed,Conyza bonarinsis could adapt itself to the changes of temperature by the addition of its POD isozymic bands,these four exotic weeds had broader niche of temperature; (2)Solidago canadensis could not endure to higher temperature,while Lantana camara to lower temperature on the basis of their POD isozymograms under 38 °C and 5 °C temperatures.