小鼠胚胎干细胞植入大鼠脑内分化的研究

DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS AFTER TRANSPLANTATION INTO THE ADULT RAT BRAIN

观察小鼠胚胎干细胞植入大鼠隔区和海马内之后的分化状况。以 SD大鼠为宿主 ,将胚胎干细胞移植入宿主隔区和海马内 ,移植后在 l、2、3、4和 8周取脑 ,冰冻切片 ,进行 Nissl染色和 M6、NSE、GFAP免疫组织化学反应。胚胎干细胞移植入大鼠隔区和海马之后 ,从第 2周开始表达 M6、GFAP、NSE等抗原 ,持续至第 4周 ,主要位于移植区内 ,较少迁移。小鼠胚胎干细胞移植入大鼠隔区和海马内之后 。

Differentiation of ES cells of mice was observed in the adult rat brain after transplantation. ES-BAL B/C cells were implanted into the hippocampus and septum of adult rat, Nissl stain, M6 antibody were used to detect and differ the implanted donor cells in the host brain. Besides, differentiation of ES cells was observed by GFAP, NSE immunohistochemistry staining in the brain. ES grafts formed a roundish mass after 2～4 weeks inside the grafts, many cells expressed M6, GFAP, NSE antigen, demonstrating that these cells have differentiated into neurons, glia and neural progenitor cells respectively. Transplanted ES cells can differentiate into neurons, glia in the rat brain.