妊娠中晚期暴露于可卡因对后代多巴胺能神经系统发育的长时程影响

LONG-TERM EFFECTS OF PRENATAL COCAINE EXPOSURE ON OFFSPRINGS' DOPAMINERGIC NERVOUS SYSTEM DEVELOPMENT

为了研究妊娠中晚期暴露于可卡因对后代多巴胺能神经系统的发育是否具有长时程影响 ,本研究建立和运用子宫内接触可卡因的小鼠动物模型 ,运用高效液相色谱结合电化学检测器 ( HPLC-EC)技术检测脑内多巴胺及其代谢产物 3,4-双羟苯乙酸和高香草酸的含量 ;采用免疫组织化学结合图象分析方法观察脑内酪氨酸羟化酶的分布与相对含量。结果表明 ,HPL E-EC检测提示妊娠期暴露于可卡因的仔鼠脑内多巴胺及其代谢产物的含量在青春前期和青春期较对照组明显升高 ;免疫组织化学方法发现妊娠期暴露于可卡因的仔鼠在青春前期与青春期脑内酪氨酸羟化酶在黑质和中脑腹侧被盖区的分布和相对含量与对照组比较有显著性差异。上述变化在成年期基本恢复正常。结论 :妊娠期暴露于可卡因对后代多巴胺能神经系统的发育具有长时程影响 。

To develop a murine model for investigating the long-term effects of prenatal cocaine exposure on offsprings' dopaminergic nervous system, the concentration of dopamine and its metabolites in brain samples was analyzed by HPLC-EC and the distribution of TH in various brain regions was observed by immunohistochemistry. Neurotransmitter analysis revealed that the concentration of DA, DOPAC, HVA in the brains of COC group pups was much higher than that in controls; the distributon of TH existed significant differents in SN, VTA region between COC group and controls. The results show that prenatal cocaine exposure can lead to a long-term effect on offsprings'dopaminergic nervous system, which might result in neurological and/or behavioral abnormalities.