鼻咽癌相关基因NGX6对鼻咽癌细胞周期的影响

NGX6 Effect on the Cell Cycle of NPC Cell Line HNE1 Cells

为了探讨鼻咽癌(NPC)候选抑瘤基因NGX6对NPC细胞的细胞周期进程及细胞周期素的影响,阐明它的作用机制,通过建立稳定表达NGX6的鼻咽癌HNE1细胞株,采用细胞免疫组织化学,流式细胞仪检测与分析细胞周期及细胞周期素的改变,用westernblot验证它对细胞周期的影响.结果显示稳定表达NGX6的HNE1细胞较对照组细胞周期中G0 G1期比例明显增加,而S期比例减少.细胞凋亡率无明显变化.流式细胞仪检测发现cyclinD1、A和E的表达明显减少,以cyclinD1的改变最为明显.Westernblot检测也发现cyclinD1的表达明显下调.以上结果说明NGX6主要通过下调cyclinD1的表达,延缓细胞周期的G1→S的进程,从而抑制NPC细胞的过度增殖.

NGX6 is a candidate tumor suppressor gene,whose expression is associated with nasopharyngeal carcinoma (NPC) tumorgenesis.Previous studies show that constitutive expression of NGX6 inhibits DNA synthesis,but its molecular mechanism remains to be elucidated.A stable NGX6transfected NPC cell line HNE1 is developed.Flow cytometry analyses indicated that the overexpression of NGX6 could delay the progression of G1→S in cell cycle,while it had no effect on the apoptosis of cells.Flow cytometry analysis and western blotting indicated that the expression of cyclinD1 significantly decreased in NGX6transfected HNE1 cells.Similar results were observed in cyclinA and cylinE.These studies provide evidence that the inhibitory effect of NGX6 on cell proliferation is mainly exerted at the G1/S boundary of the cell cycle by downregulating the expression of cyclin D1.