摘要
采用固体膜进行TSST 1的产毒培养,然后从金葡菌产毒产物中经CM纤维素离子交换层析、SephadexG 75、SephacrylS 200三步纯化,成功地获得了纯度为95.3%的毒素,SDS PAGE呈现单一条带,与其对应抗血清有免疫学特异反应,不含核酸、糖和其它肠毒素等物质.与同类方法相比,该方法步骤少、快速、简便.对其体外抗肝癌活性进行了分析,首次证明即使TSST 1的浓度为10-8g L仍然对人肝癌细胞有显著抑制作用.
A simple and efficient three step procedure was developed to purify TSST1 after culturing the toxinproducing bacteria on membrane.The purification procedure involved ionexchange chromatography on CM cellulose,gel filtration on Sephadex G75 and Sephacryl S200.TSST1 with high purity was obtained.The purified toxin is a simple protein with a molecular weight of 22 kD as determined by SDSPAGE.Other material such as nuclear acid and staphylococcal enterotoxins are not contained in the purified products.Specific reaction of purified TSST1 is demonstrated by immunodiffusion test.Our improved purification procedure is simple, more rapid and convenient than previously reported methods.The antitumor activity of TSST1 was also analysed.It was demonstrated for the first time that even 10-8?g/L TSST1 can notably inhibit the tumor growth of human hepatocellular carcinoma in vitro.
出处
《生命科学研究》
CAS
CSCD
2002年第4期347-350,共4页
Life Science Research
基金
国家自然科学基金资助(30171091)