杉木对炭疽病的抗性与苯丙氨酸解氨酶的关系

RELATION BETWEEN CHINESE FIR RESISTANCE TO GLOMERELLA CINGULATA AND PHENYLALANINE AMMONIA LYASE (PAL)

接种前的杉木 (Cunninghamialanceolata)针叶中 ,苯丙氨酸解氨酶 (PAL)比活力与抗感病性之间不呈规律性相关 .不同抗病性的杉木接种炭疽菌 (Glomerellacingulata)后 ,PAL比活力均上升 ,接种 5d后 ,抗病性强的杉木PAL比活力比抗病性弱的杉木高 .接种前以及接种后的各个时间段内 ,木质素含量都是抗病杉木比感病杉木高 .绿原酸的含量与杉木的抗病性之间不呈规律性的相关 ,从绿原酸对炭疽菌菌丝生长影响的测定结果可知 ,杉木针叶的绿原酸浓度不足以构成对炭疽菌的影响 .可见 ,接种后针叶的PAL比活力以及接种前、后针叶的木质素含量可作为衡量杉木抗病性的指标 .图 3表 2参

The study results showed that the specific activity of phenylalanine ammonia lyase (PAL) in the healthy leaves of Chinese fir (Cunninghamia lanceolata) was not relative to the host resistance to Glomerella cingulata, while the PAL specific activity increased in the different resistant hosts after inoculation, and the PAL specific activity in the resistant host was higher than those in the susceptible one after inoculation for 5 d. The contents of lignin in the resistant host was higher than that in the susceptible one before and after inoculation. The content of chlorogenic acid in Chinese fir was not relative to the resistance to the Glomerella cingulata. So, the PAL specific activity after inoculation and the content of lignin before or after infection in leaves could be used as the criterion to evaluate the resistance to the disease. Fig 3, Tab 2, Ref 14