心肌细胞凋亡模型的建立及检测方法

Built of model for apoptosis of cardiac muscle cell and its detecting

目的:研究诱导体外培养心肌细胞凋亡的方法。方法:体外培养的新生大鼠心肌细胞置于95％N2,5％CO2孵箱中一定的时间,细胞模型形成缺氧损伤,分别用HE染色,电镜,TUNEL法,流式细胞仪技术检测凋亡发生的情况。结果:在缺氧培养一定时间后,用上述方法均观察到缺氧诱导的心肌细胞凋亡。结论:缺氧一定的时间可以诱导体外培养的心肌细胞凋亡;检测凋亡需结合形态学及定量测定作全面分析。

Objective: To study the built of model for apoptosis of cardiac muscle cell and its detecting. Methods: Apoptosis of cardiac muscle cell from neonatal rat was induced under 95% N2 and 5% CO2 in incubated box. HE staining、 transmission electron microscopy、 TUNEL and flow cytometer were taken for observing after 16 hours culturing. Results: Apoptosis of cardiac muscle cell was observed by all four methods. In contrast, cardiac muscle cell in control group was normal. Conclusion: Model for apoptosis of cardiac muscle cell can built in hypoxia culture. All four methods detecting can detect the apoptosis of cardiac cell and each method has his strong point.