对氧磷酯酶基因多态性与糖尿病肾病的关系

Relationship between paraoxonase 2 A148G polymorphism and diabetic nephropathy

目的 探讨对氧磷酯酶2(PON2)基因A148G多态性与糖尿病肾病的关系。方法(1)用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析法探查PON2基因A148G多态性在正常对照组、单纯2型糖尿病组、糖尿病肾病组中的基因频率分布;(2)放射免疫法检测血清免疫反应性胰岛素(IRI)、C肽(C-P)水平。结果(1)糖尿病肾病组GG基因型和G等位基因频率明显高于单纯2型糖尿病组(x2=4.26 P<0.05,X2=4.89 P<0.05)和正常对照组(x2=4.79 P<0.05,x2=5.49P<0.01);(2)基因型为GC的糖尿病患者空腹血糖浓度高于基因型为GA和AA的糖尿病患者的空腹血糖浓度(F=3.90 P<0.05,F=4.23 P<0.05);(3)Logistic回归分析表明 GG基因型是糖尿病肾病的独立变异危险因素(P<0.05)。结论PON2基因多态性与糖尿病肾病的发生有关。

Objective To determine whether the paraoxonase2 (PON2) gene A148G polymorphism is associated with the pathogenesis of nephropathy in type 2 diabetes mellitus patients (DN) . Methods PON2 genotype distribution and allele frequency were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) . Serum immune reactive insulin (IRI) and C-peptide (C-P) concentrations were measured by radioimmunity. Results GG genotype and G allele frequency were higher in DN group than that in simple type 2 diabetes mellitus group (x2 =4. 26 P < 0. 05, x2 =4. 89 P < 0. 05) and normal control group(x2 =4. 79 P < 0. 05, X2 = 5.49 P <0.05). The subjects with GG genotype had higher fasting plasma glucose concentration than those with GA and AA genotypes ( F = 3. 90 P < 0. 05, F = 4.23 P < 0. 05). Logistic regression analysis revealed that GG genotype was an independent risk factor of diabetic nephropathy( P < 0. 05) . Conclusion PON2 A148G polymorphism is associated with the pathogenesis of DN.