RP-HPLC测定大鼠血浆中水飞蓟宾的浓度

DETERMINATION OF SILIBININ CONCENTRATION IN RAT PLASMA BY RP-HPLC

目的 测定大鼠血浆中水飞蓟宾的浓度。方法 用RP -HPLC。样品以叔丁基甲醚提取 ,色谱柱为PoresphereC18柱 (4.0mm× 2 5 0mm ,5 μm) ,流动相为甲醇 水 0 .0 5mol·L-1磷酸二氢钾 (2 0∶10∶7) ,流速为 1.0ml·min-1,检测波长为 2 88nm ,温度为室温。结果 大鼠血浆中成分对水飞蓟宾的测定无干扰 ;在 0 .0 0 6 1～ 3.12 5 μg·ml-1浓度范围内呈现良好的线性关系 ,r=0 .9992 ;血浆中水飞蓟宾的最低检测浓度为 6ng·ml-1,平均回收率为10 7.0 0 % ,日内RSD≤ 8.19% ,日间RSD≤ 13.78%。结论 该法样品处理简便 ,灵敏度高 ,结果准确。

OBJECTIVE To establish an RP-HPLC method for silibinin determination in rat plasma. METHODS The column was Poresphere C 18 (4.0 mm×250 mm,5 μm) and the mobile phase was methanol--water-0.05 mol·L -1 potassium dihydrogen phosphate(20∶10∶7) with the flow rate of 1.0 ml·min -1 .The detection wavelength was set at 288 nm. RESULTS Silibinin was separated and no interference was found. A good linear relationship was obtained between 0.006-3.125 μg·ml -1 ( r = 0.9992 ). The limit of detection was 6 ng·ml -1 . The mean recovery was 107.00% , The within a day RSD and the between days RSD were less than 8 19% and 13.78 %. CONCLUSION This method is simple, rapid, sensitive, accurate with a wide linear range and suitable for the analysis of silibinin-containing biological samples.