养阴益气活血方活性物质对脑缺血性损伤的保护作用

Experimental Studies on the Mechanism of Yangyin Yiqi Huoxue Recipe(养阴益气活血方) Treated on Stroke Caused by Cerebral Ischemia

目的 :探讨养阴益气活血方活性物质对脑缺血性损伤保护作用的研究。方法 :采用四血管阻断法造成大鼠全脑缺血模型 ,观察脑匀浆液丙二醛 (MDA)和超氧化物歧化酶 (SOD)活性、一氧化氮 (NO)、内皮素 (ET)、环磷酸腺苷 (cAMP)及血浆皮质醇 (F)、全血谷光甘肽过氧化物酶 (GSH PX)活力的含量变化及相应的病理改变。结果 :养阴益气活血方在显著降低缺血再灌注大鼠脑组织中MDA、NO、ET、cAMP含量的同时 ,提高SOD、GSH PX的活性。结论 :养阴益气活血方能抑制脂质过氧化反应 ,提高机体抗氧化能力 ,对微血管和细胞损伤产生保护作用 ,因此对脑缺血再灌注损伤有显著的保护作用。

Objective:To explore the mechanism of Yangyin Yiqi Huoxue recipe(YYYQHXR,养阴益气活血方)treated on stroke caused by cerebral ischemia. Method:Rat model of cerebral ischemia is prepared by four vessel interdiction,observe the contents of malonyldialdehyde (MDA),superoxide dismutase(SOD),nitric oxide(NO),Endothelin (ET),cyclic AMP(cAMP),fludrocortisone (F)in encephalon tissue serosity. We can also approach to the variation of glutathine peroxidase(GSH PX)activity and its corresponding pathologic alteration. Result:YYYQHXR can obviously decrease the contents of MDA,NO,ET,cAMP and simultaneously increase the activity of SOD and GSH PX. Conclusion:YYYQHXR can inhibit over oxidation of lipid,promoting anti oxidative,and significantly protect cerebral against injury caused by cerebral ischemia reperfusion injury.