散血明目片抑制积血所致PVR的实验研究

Experimental Study of SanXue MingMu TABLET on PVR Induced by Hematocele

目的 :研究散血明目片对兔玻璃体积血所致PVR的影响。方法 :采用自体血造兔右眼玻璃体积血模型 ,分别于造模后 4周、8周观察增殖膜分级 ,采用酶联免疫双抗体夹心法 (ELISA)检测积血玻璃体中TNF α、IL 6含量。结果 :玻璃体积血 4周 ,玻璃体内增殖不明显 ;TNF α、IL 6含量 :散血明目片治疗组与血塞通对照组之间有显著性差异 (P <0 .0 5 ) ,与模型组之间TNF α有显著性差异 (P <0 .0 1)、IL 6无显著性差异 (P >0 .0 5 ) ,与空白组有显著性差异 (P <0 .0 5 ) ,维持了TNF α、IL 6的高表达 ;8周 ,散血明目片组增殖最轻 ;TNF α、IL 6含量明显降到低水平 ,与血塞通对照组有显著性差异 (P <0 .0 5或P <0 .0 1) ,与模型组之间有显著性差异 (P <0 .0 1) ,与空白组无显著性差异 (P >0 .0 5 )。结论 :散血明目片在玻璃体积血的早期能维持玻璃体内TNF α、IL 6的高表达 ,在积血的中晚期能够降低玻璃体内的TNF α、IL 6的含量。因此 ,散血明目片不仅可以加速玻璃体积血的清除 ,并且可以抑制玻璃体内细胞增生的启动、细胞的移行和增殖 ,从而预防PVR的发生发展。

Objective:To study the effection on PVR induced by vitreous hemorrhage of rabbits with SanXue MingMu Tablet (SXMMT). Methods:Using models of vitreous hemorrhage in rabbits' right eyes by autoblood,and observing proliferation membran grade separately and detecting the contents of TNF α and IL 6 in hematocele vitreum by enzyme\|linked immunoads cordent assay (ELISA) after four weeks and eight weeks of model built. Results:After four weeks,the proliferation in vitreum isn't obvious,there are significant difference in the TNF α and IL 6 levels between the SXMMT treated group and the XSTT control group ( P <0.05).In the TNF α and IL 6 levels:The TNF α levels were significantly different( P <0.01),but the IL 6 levels weren't significantly different between the model control group and the SXMMT group ( P >0.05),between the SXMMT group and the blank group there were significantly difference ( P <0.05),TNF α and IL 6 maintain high expression.After eight weeks,the most lightly proliferation in the SXMM group and the evidently low Decreasing of the TNF α and IL 6 were observed,there was significant difference with the XST control group( P <0.05 or P <0.01) and the model group( P <0.01),but there was no significant difference with the blank group( P >0.05).Conclusions:The high expression of IL 6 and TNF α could be maintained by SXMMT in vitreum during earlier period of Vitreous hemorrhage and their levels were decreased during the mid and late period.Therefore,SXMMT can not only accelerate the disaperence of vitreous hemorrhage but also prevent cellular proliferation occurrence,cellular migration and proliferation,so as to prevent the occurrence and progress of PVR.