高压氧对慢性肝炎患者体液免疫及肝组织中HBsAg、HBcAg的影响

The effects of hyperbaric oxygenation on the humoral immunity and the expression of HBsAg、HBcAg in the liver tissues of chronic hepatitis patients

目的 观察慢性肝炎 (CH)高压氧 (HBO)治疗前后患者的体液免疫功能和肝组织中 HB-s Ag、HBc Ag的变化。方法 用单人纯氧舱治疗 (0 .2 5 MPa,1.5 h/ d,10 d/ course,6 courses) 6 0例 CH,用 Array TM Protein System Array16 0测定治疗前后静脉血α-白蛋白、γ-球蛋白 ,Ig A、Ig G、Ig M,补体C1 、C4 ;肝穿刺活检 ,SP法检测肝组织中 HBs Ag、HBc Ag。结果 HBO治疗后 CH患者静脉血γ-球蛋白、Ig A、Ig G、Ig M、补体 C1 、C4 明显降低 ,α-白蛋白明显升高 ,治疗前后差异显著 (P<0 .0 5 ) ;CH患者肝组织中 HBs Ag、HBc Ag变化不明显 ,治疗前后差异不显著 (P>0 .0 5 )。结论 HBO治疗 CH,可降低患者的体液免疫反应 ,对 CH肝组织中 HBs Ag、HBc Ag抑制作用不明显。

Objective To observe the changes of humoral immunity and the expression of HBsAg?HBcAg in the liver tissues of chronic hepatitis before and after the hyperbaric oxygenation(HBO) treatment. Methods 60 cases of chronic hepatitis were treated with HBO(0.25 MPa,1.5 h/d, 10 d/course) for 6 treatment courses. The immunity factors(α albumin?γ globulin,IgA?IgG?IgM,C 1?C 4) in the serum of the patients before and after treatment were measured by Array TM Protein System Array 160. HBsAg and HBcAg were detected in the liver tissues using immunocytochemistry methods(SP). Results After treatment with HBO,γ,IgA,IgG,IgM,C 1,C 4 in the serum were remarkable reduced and α were remarkable increased( P <0 05). There were no significant changes in HBsAg and HBcAg in the liver tissues of the patients with CH before and after the HBO treatment( P >0 05). Conclusions For patients with chronic hepatitis, the HBO treatment may reduce the humoral immunity reaction, but could not eliminate HBsAg and HBcAg in the liver tissues.