益肝浓缩煎剂与强肝胶囊抗大鼠肝纤维化作用实验研究

Experimental Study of Effect of Concentrated Yigan Decoction and Qiangan Capsule on Hepatic Fibrosis in Rats

目的:研究益肝浓缩煎剂、强肝胶囊对大鼠肝纤维化治疗作用,并对其机制进行初步探讨。方法:CCl_4诱导大鼠肝纤维化模型,造模结束后分别予相应药物进行治疗,持续10周,测定肝组织羟脯氨酸(Hyp)、丙二醛(MDA)含量,光镜观察肝脏病理形态变化。结果:与模型自然恢复组相比,益肝浓缩煎剂、强肝胶囊治疗组Hyp、MDA显著降低,胶原沉积明显减轻。结论:益肝浓缩煎剂、强肝胶囊对大鼠肝纤维化具有明确治疗作用,可促进胶原纤维降解,使早期肝纤维化逆转。其疗效作用机制可能与抗脂质过氧化有关。

Objective: To study the therapeutic effects of Concentrated Yigan Decoction and Qianggan Capsule on hepatic fibrosis in rats as well as their relative mechanisms. Methods: Hepatic fibrosis in rats was induced by CCl4 and Concentrated Yigan Decoction or Qianggan Capsule was given after model establishment. The administration of the two formulas lasted for 10 weeks. Liver hydroxyproline (Hyp) and malondialdehyde (MDA) level were determined and histopathological changes were observed by optical microscopy. Results: In Concentrated Yigan Decoction and Qianggan Capsule therapeutic group, the contents of Hyp and MDA as well as degrees of hepatic fibrosis were markedly reduced repectively compared with those of model of natural recovering group. Conclusion: Concentrated Yigan Decoction and Qianggan Capsule both had remarkably therapeutic effects on hepatic fibrosis of rats. They could resolve the collagens accumulated in liver of rats and hepatic fibrosis could be reversible. Antiperoxidation of lipid might be related to the two formulas' therapeutic effects.