三种环境内分泌干扰物对前列腺癌细胞PC-3增殖的影响

Effects of Environmental Endocrine Disruptors on the Proliferation of PC-3 Cells

[目的 ]通过观察环境内分泌干扰物对 壬基酚 (n 4 noniphenol,NP)、双酚A(bisphenolA ,BisA)和邻苯二甲酸二丁酯 (dibutylphthalate ,DBP)对前列腺癌细胞PC 3增殖的影响 ,探讨近几年来前列腺癌发病率增加的原因。 [方法 ]前列腺癌细胞株PC 3在RPMI 164 0培养液中采用开放式单层贴壁培养 ,培养条件为 3 7℃ ,5 %CO2 ,10 0 %相对饱和湿度 ,采用MTT法、3 H TdR掺入法及流式细胞术对PC 3细胞的增殖情况进行分析。实验设溶剂对照组及每种受试物各四个剂量组。 [结果 ]NP、BisA和DBP均可促进PC 3细胞增殖和细胞DNA合成并推进G0 /G1期细胞进入S期 ,提高细胞增殖指数 ;随着培养时间的延长至 96h ,在较低浓度条件下 ,0 5 μmol/LNP、0 .5 μmol/LBisA和 2 5 μmol/LDBP也能明显促进PC 3细胞增殖 (P <0 0 5 ) ,且其促进PC 3细胞增殖效应存在剂量 效应关系和时间 效应关系。 [结论 ]NP、BisA和DBP可促进前列腺癌细胞株PC 3的增殖 ,提示环境中内分泌干扰物污染增加 ,可能是近几十年来前列腺癌发病率上升的原因之一。

This study was designed to explore the role of environmental disruptors(n 4 nonyphenol,bisphenol A and dibutylphalate) in increasing the incidence of prostate cancer in recent years due to their effects on the proliferation of prostate cancer PC 3 cells. Prostate cancer PC 3 cells were grown in RPMI 1640 medium containing 5% dextran charcoal stripped FBS.Cells were cultured at 37℃ with 5% CO 2 in a humidified incubator. The respective test compounds were added into fresh medium and the control cells received only the vehicle(ethanol). The proliferation of PC 3 cells was measured by MTT assay. The DNA synthesis of PC 3 cells was detected by 3H TdR incorporation assay and the cell cycle of PC 3 cells was analyzed by flow cytometry. Comparing with the control cells,this study demonstrated that the proliferation of PC 3 cells receiving n 4 nonyphenol (7 5 μmol/L,24 h) or bisphenol A (7 5 μmol/L,24 h) or dibutylphthalate (75 μmol/L,48 h) treatment was markedly enhanced and the results showed time dependent and dose dependent relationship. [Conclusion] The all tested chemicals enhanced the proliferation of human prostate cancer PC 3 cells in vitro. The results implied that the increasing in the incidence of breast cancer in recent years might be partly accounted for environmental pollution and the augment of environmental disruptors.