摘要
In order to refine the products of wheat germ agglutinin(WGA),several ion exchangers,such as D261,732,DEAE-cellulose-32 and CM-cellulose-32, have been used to removed proteins and pigments with different colors,such as brown,red,yellow,green and black,in the extracts of wheat germ.The WGA obtained from this procedure has higher hemagglutination activity than that available from sigma Co. The minimum hemagglutination dose of the purified WGA for an equal volume of a 2% type A red blood cells is 4μg/ml.WGA is a mixture of isolectins with different isoelectric points,5.9,6.2 and 6.8.Their molecular weight identified by SDS-polyacrylamide gel electrophoresis are 15,000 dalton,18,000 dalton and 35,000 dalton,respectively.
In order to refine the products of wheat germ agglutinin (WGA) , several ion exchangers, such as D261,732, DEAE - cellulose -32 and CM - cellulose - 32, have been used to removed proteins and pigments with different colors, such as brown, red, yellow, green and black, in the extracts of wheat germ. The WGA obtained from this procedure has higher hemagglutination activity than that available from sigma Co. The minimum hemagglutination dose of the purified WGA for an equal volume of a 2% type A red blood cells is 4μg/ml. WGA is a mixture of isolectins with different isoelectric points, 5. 9, 6. 2 and 6. 8. Their molecular weight identified by SDS-polyacrylamide gel electrophoresis are 15 ,000 dalton , 18,000 dalton and 35,000 dalton, respectively.
