摘要
With cellulose as carrier, immobilized thermolysin HE Ⅰ and HE Ⅱ were prepared by diazo coupling or glutaraldehyde crosslinking reaction. Using casein as a substrate, the activity recovery of immobilized thermolysin HE Ⅰ and HE Ⅱ reach 34. 596 and 29%, respectively. Some factors which affect the activity of the immobilized thermolysin such as temperature, medium PH, EDTA and calcium acetate were studied. At the same time, the thermal stability and storage stability Of the immobilized thermolysin were also investigated.
ＳＴＵＤＹＯＮＩＭＭＯＢＩＬＩＺＥＤＴＨＥＲＭＯＬＹＳＩＮＷＩＴＨＣＥＬＬＵＬＯＳＥＡＳＣＡＲＲＩＥＲＴａｏＧｕｏｌｉａｎｇ；ＺｈｕｏＲｅｎｘｉ；ＣｈｅｎＺｈｅｎｈｕａ；ＺｈｏｕＸｉａｏｑｉｎ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔｒｙ，ＷｕｈａｎＵ...
