氧化锆及铈-锆复合氧化物的阴离子交换和配体交换色谱性能

Ion-exchange and Ligand-exchange Properties of Zirconia and Zirconia-ceria Stationary Phases

用溶胶 -凝胶法制备了 Zr O2 和 Zr O2 -* Ce O2 2种球形色谱固定相 ,研究了无机阴离子及取代苯甲酸化合物的色谱保留行为。当流动相 p H值小于氧化物的零电荷点时 ,固定相表现出明显的阴离子交换色谱性能 ,几种无机阴离子的洗脱顺序为 I- 、Br- 、NO- 3 、NO- 2 、Br O- 3 。由于配体交换作用的存在 ,苯甲酸及其衍生物在 Zr O2 和 Zr O2 -Ce O2 固定相上有较强保留 ,当流动相中存在竞争配体时 ,其保留值下降。采用醋酸盐缓冲液 ,苯甲酸及其衍生物能获得较为合适的保留值。

Zirconia and zirconia-ceria microspheres were synthesized as stationary phases for HPLC by a sol-gel process. The chromatographic behavior of inorganic anions and benzoic acid derivatives was systematically investigated. Zirconia and zirconia-ceria exhibit anion-exchange property only in the pH value below their zero change points. The elution order of five inorganic anions are I -, Br -, NO - 3, NO - 2, BrO - 3. Benzoic acid derivatives showed a more retention value on zirconia and zirconia-ceria mainly by ligand-exchange interaction, and the retention values were attenuated by the addition of a competing Lewis bases to the mobile phase. Moderate retention of benzoic acid derivatives on zirconia and zirconia-ceria can be obtained by using acetate buffer as a mobile phase.