壳聚糖的溶菌酶降解

Hydrolysis of chitosan with lysozyme

用粘度法和还原糖法研究了溶菌酶降解壳聚糖过程中温度、pH值、时间、酶浓度、底物浓度以及金属离子对降解速度的影响。比较合适的降解条件是 :温度 45℃ ,pH值 5 0 ,适当增大酶浓度和底物浓度能够加速壳聚糖的降解 ,在一定的底物浓度下溶菌酶降解壳聚糖的反应不遵循简单的一级反应动力学。一定浓度的Cu2 + 、Zn2 + 、Ba2 + 、Cr3 + 能够促进壳聚糖在溶菌酶作用下的降解 ,而K+ 、Ca2 + 和高浓度的Zn2 + 会抑制酶活力的发挥。

The effects of reaction temperature, pH, time, substrate and enzyme concentrations, as well as metal ions on the hydrolytic rate of chitosan by lysozyme were investigated. The optimum temperature and optimum pH were 45?℃ and 5.0 respectively. Increasing the concentrations of substrate and enzyme solutions could accelerate the hydrolysis of chitosan. The depolymerization of chitosan didn't conform to the simple enzymic kinetics model within certain substrate concentrations. A certain concentration range of Cu 2+, Zn 2+, Ba 2+ or Cr 3+ can greatly improve the enzymic activity of lysozyme, while K +, Ca 2+ and a high concentration of Zn 2+ will inhibit the enzymic activity.