使用手性冠醚及手性柱的HPCE和HPLC法拆分伯胺类药物对映体的对比研究

Study on the Chiral Separation of Drugs Having a Primary Amino Group by HPCE and HPLC Utilizing Chiral Crown Ethers and Column

报道了在缓冲液中添加 1 8-冠 - 6 -四甲酸 (1 8C6 H4 )的高效毛细管电泳法(HPCE)对 8个含氨基的药物进行拆分 ,低 p H值 (2 .0 6 )和较高浓度的 Tris缓冲液(2 0 mmol· L- 1)可加快手性分离。用手性柱 Crownpak CR(+)的高效液相色谱(HPLC)法对与前相同的 8个含氨基药物进行拆分 ,甲醇浓度增大 ,分离度 RS值有所减小。实验结果还表明 ,使用 HPCE法拆分的各物质 RS值大于使用 HPLC法拆分相应物质的 RS值。

Chiral separation of amino-drugs by high performance capillary electrophoresis was achieved by the addition of a chiral crown ether,18-crown-6 tetracarboxylic acid (18C6H 4). Low pH values (pH 2.06) and relatively high Tris concentration (20 mmol·L -1) were effective for the fast enantiomer separation. Enantiomer separation of the same amino drugs was also investigated by high performance liquid chromatography (HPLC) with a chiral Crownpak CR(+) column. By increasing methanol content, the enantioselectivity (resolution) was decreased. Rs value (HPCE) was bigger than Rs value (HPLC).