一氧化氮合成阻滞大鼠模型血浆一氧化氮变化及益气化瘀法干预作用

Nitrogen Oxide Synthesis to Blood Change of Plasma Nitrogen Oxide of Rat Model and Intervention of Yiqi Huayu Principle

目的 探讨一氧化氮 (NO)在妊娠高血压综合征及胎儿宫内发育迟缓发病中的作用及益气化瘀法提高胎盘血供的内在机制。 方法 采用 L-精氨酸甲酯 (L- NAME)于妊娠第 10 d起皮下注射制作模型 ,黄芪、丹参复方成分及硝酸甘油干预 ,于妊娠 12 d起测尾动脉血压 ,考马斯亮兰法检测尿蛋白含量 ,妊娠第 18d用亚硝酸根还原酶法检测血浆NO水平 ; 结果 模型组 NO含量明显降低 (P<0 .0 1) ,经黄芪丹参复方成分治疗后 ,血浆 NO水平升高 (P<0 .0 1) ,而阳性组 NO含量稍有升高 ,但差异无统计学意义。 结论 以益气化瘀立法的黄芪丹参复方成分舒张血管、降低外周阻力 ,改善胎盘循环的作用可能是通过促进内皮细胞分泌

Objective To explore the effect of NO in pregnant hypertansion syndrome and fetal intrauterine growth retardation as well as the intrinsic mechanism of YiqiHuoxue principle(YQHYF)in increasing placental blood supply. Methods Applying L-Arginine methylester(L-NAME) on day 10 of model animals' pregnancy, the ingredient of milkvetch and red sage compound recipe and netroglycerine were used to intervene. On day 12 of pregnancy, blood pressure of caudal artery was measured. Urinary protein level was determined, NO level was measured on day 18 with silver nitrite reductase assay. Result The NO level of model group obviously lowered(P<0.01). After being treated with milkvetch-red sage compound recipe, plasma NO level raised(P<0 01),while in positive group, the NO level also slightly increased but without statistical significance. Conclusion Dilation of the blood vessel, lowering of the peripheral resistance, improve ment of the placental circulation of YQHYF with milkvetch-red sage was supposed to realize by promotion of the secretion of NO by endothelial cell.