三甲氧苄嗪对心肌缺血/再灌注损伤的保护作用

Protective effects of trimetazidine on myocardial ischemia-reperfusion injury

目的 :探讨三甲氧苄嗪增补于停搏液中对缺血 /再灌注离体鼠心的保护作用。方法 :将 2 4只 Wistar大鼠随机分为三甲氧苄嗪组 (A)和对照组 (B)。离体鼠心在改良的 L angendorff- Neely灌注模型上预灌注 30 min,停搏 12 0min、再灌注 30 min。缺血前及再灌注期间测定血流动力学指标、心肌酶 (CPK,L DH )、心肌超氧化物歧化酶 (SOD)、过氧化脂质 (L PO)含量、心肌 ATP水平。电镜观察心肌超微结构。结果 :再灌注后 ,A组心功能、心肌超微结构的改善 ,明显优于 B组 ;心肌酶 (CPK ,L DH)、L PO含量显著低于 B组 (P<0 .0 1) ;SOD含量和 ATP水平显著高于 B组(P<0 .0 1)。结论 :三甲氧苄嗪增补于停搏液中可显著减轻心肌缺血 /再灌注损伤 。

AIM:To study the protective effects of trimetazidine on myocardial ischemia reperfusion injury. METHODS:The study consisted of two groups of rats. Group A( n =12),received cardioplegia with trimetazidine. Group B( n =12),received same cardioplegia only. Isolated rat hearts were subjected to preperfusion for 30 min,ischemia for 120 min and reperfusion for 30 min using a modified Langendorff Neely model with modified Krebs Henseleit buffer at 37℃. The hemodynamic parameters,myocardial enzyme(CPK,LDH),myocardial superoxide dismutase(SOD),myocardial ATP level,myocardial lipid peroxide(LPO) and myocardial ultrastructure were measured before ischemia and during reperfusion. RESULTS:Recovery of cardial function and myocardial ultrastructure in Group A was significantly better than Group B. Myocardial enzyme (CPK,LDH) and LPO in group A was significantly lower than in Group B( P <0.01). SOD was significantly higher than Group B( P <0.01). Myocardial ATP level was significantly higher than Group B( P <0.01).CONCLUSION:Trimetazidine supplement in cadioplegia significantly alleviated reperfusion mediated myocardial injury after global ischemia,suggesting good myocardioprotective actions.