HPLC法测定克癃胶囊中补骨脂素、异补骨脂素的含量

Determination of Psoralen angelicin and Isopsoralen angelicin in KELONG Capsule by HPLC

目的 用 HPL C法测定克癃胶囊中补骨脂素、异补骨脂素的含量。方法 色谱条件 :色谱柱 :C1 8柱 (2 5 0× 4.6mm,5μm) ,流动相 :甲醇 -水(60∶ 40 ) ,检测波长 2 45 nm。结果 补骨脂素在 0 .0 964～ 0 .482 μg,异补骨脂素在 0 .0 868～ 0 .43 4μg范围内呈良好线性关系。平均回收率分别为10 0 .7%;98.9%,RSD分别为 1.3 9%;2 .11%。

OBJECTIVE To determine the content of psoralen angelicin and isopsoralen angelicin in KELONG Capsule by HPLC. METHODS Separation was achieved on a C 18 column(250×4 6mm,5μm).The mobile phase was metanol water(60∶40),the wavelength of the UV detector 245nm.RESULTS Linear range of psoralen angelicin and isopsoralen angelicin were 0.0964～0.482μg and 0 0868～0 434μg,The average recovery were 100.7% and 98.9%(RSD were 1.39% and 2.11%),respectively.CONCLUSION The method was accurate and reliable.