苍术超临界CO_2萃取物的质量标准研究

Studies on quality standard of tractylon from supercritical CO_2 extraction

采用薄层色谱法(TLC),以石油醚(60～90 ℃)为展开剂,ρ=5％对二甲氨基苯甲醛的ρ=10％硫酸溶液为显色剂,对苍术超临界CO2萃取物中的苍术素进行鉴别。并建立毛细管气相色谱测定苍术素含量的方法。结果表明,苍术素在ρ=20.478～204.78μg/mL范围内,苍术素峰与内标峰面积之比值与浓度呈良好的线性关系,r=0.9996。此法的精密度高,重复性和稳定性好,可作为苍术质量控制的手段之一。

In order to establish the quality standard for Atractylodes, qualitative and quantitative analyses of tractylon were carried out. TLC was used as a qualitative identification method of tractylon. The tractylon content was determined by capillary gas chromatograph. The results show that the capillary gas chromatograph method is accurate, reliable and well reproducible. This method can be applied in the quality control of Atractylodes.