三种不同DNA提取法对血链球菌随意引物PCR鉴定影响的研究

Influence of different DNA extractions on the identification of streptococcus sanguis group by arbitrary primed polymerase chain reaction

目的 :研究不同 DNA提取法对随意引物聚合酶链反应 (AP- PCR)鉴定血链球菌的影响。方法 :采用 3种DNA提取法 ,利用 2 5碱基引物 5′AAG AGA GGA GCT AGC TCT TCT TGG A3′对不同菌种的血链球菌 DNA进行 AP- PCR。结果 :不同菌种的血链球菌 DNA经 AP- PCR扩增后产生不同的 DNA片段 ;用不同方法提取同种血链球菌 DNA可以得到相同的 DNA片段。结论 :不同

Objective: To assess the influence of different DNA extractions on the identification of streptococcus sanguis group (SSG) species by arbitrary primed polymerase chain reaction (AP PCR). Methods: AP PCR was used to distinguish SSG species by designing 25bp arbitrary primer 5′ AAG AGA GGA GCT AGC TCT TCT TGG A 3′, and the genomic DNA was extracted by 3 methods. Results: There were great differences in the main band of DNA polymorphism among SSG species. The similar band could be got from the different DNA extractions in the same species. Conclusion: Different DNA extractions have no influence on the identification of SSG.