昆明种小鼠正常行为发育指标探讨

Behavioral teratogenic test in KunMing mouse

目的 探讨昆明种小白鼠生长和行为发育指标 ,为小鼠行为毒理学测试方法标准化提供依据。方法 选用 44只昆明种孕鼠 ,自然分娩 ,观察其哺乳情况及仔鼠耳廓分离、上下牙萌出、张耳、睁眼、睾丸下降、阴道开口等生理发育指标、平面翻正、前肢悬挂、爬行、空中翻正及负重游泳等神经反射和运动协调功能。结果 每只孕鼠平均产仔 (11 93± 2 71)只 ,仔鼠出生存活率为 86 0 9% ,哺育存活率为 6 6 5 1%。结论 昆明种小白鼠生理发育指标与B6C3F1小鼠及CD 1小鼠各发育指标大致相当 ,平面翻正指标差别较大 ,可能与观察方法或小鼠品系有关。该试验为进一步的研究提供了参考依据。

Objective To investigate the indices of behavioral development in KunMing mouse and provide evidence for standardizing test methods for behavioral toxicology. Methods Forty-four pregnant KunMing mice were selected for study. After natural delivery, lactating condition and growth and development of the newborns were examined. The research tested the indices of physiological development such as pinna detachment, ear unfolding, eye opening, movement coordination function such as surface righting,fore limb hanging test, crawling, air righting etc. Results Every pregnant mouse had 11.93±2.71 newborns on average. The survival rate on the 4th and the 21st day was 81.09% and 66.51% respectively. Conclusion The indices of physiological development in KunMing mice are equivalent to that of B 6C 3F 1 and CD-1 mice. There is, however, significant difference in surface righting, which may result from different investigation methods or different kinds of mice. This research provides evidence for further researches.