草乌中生物碱含量测定方法的研究

The Method for Determination of Aconitum Alkaloids in Radix Aconiti Kusnezoffii

研究草乌中乌头类生物碱的含量测定方法,并对草乌生药进行测定。总生物碱的含量采用容量法和酸性染料比色法测定;容量法采用硫酸回滴法,以甲基红-溴甲酚绿为指示剂;酸性染料比色法采用溴甲酚绿法,检测波长416 nm。酯型总生物碱的含量采用异羟肟酸铁比色法测定。三种双酯型生物碱的含量采用高效液相色谱法测定,固定相为C18,流动相为乙腈:缓冲液(冰醋酸-三乙胺,pH6.25)=71:29,检测波长235 nm。

The methods for the determination of Aconitum alkaloids in Radix Aconiti Kusnezoffii were established. Volumetric analysis with the indicator of methyl red-bromcresol green and colonmetric analysis with acid dye of bromecresol green under the wavelength of 416nm were used for the assay of total alkaloids. Colonmetric analysis of hydroxamic acid-Fe was for the assay of total ester-diterpene type Aconitum alkaloids. Three kinds of diester-diterpene type Aconitum alkaloids (aconitine, hypaconitine and mesaconitine) were determined by HPLC method.The HPLC system consisted of C18 as the stationary phase and acetonitrile;buffer (acetic acid-triethylamine,pH6.25) = 71:29 as the mobile phase.The wavelength was 235 nm.