白藜芦醇对大鼠脊髓损伤后受损局部Ca^(2+),Mg^(2+)-ATP酶活性的影响

Effects of Resveratrol on Ca^(2+) , Mg2^(2+)-ATPase Activities after Spinal Cord Trauma in Rats

目的:探讨白藜芦醇(resveratrol,Res)对脊髓损伤(SCI)后受损部位局部Ca2+,Mg2+-ATP酶活性的影响,分析SCI后局部微环境改变及Res的作用。方法:采用重物下落撞击法制备成年大鼠的脊髓损伤模型,于损伤后即刻腹腔注射给予Res 50 mg/kg,100 mg/kg或甲基强的松龙(MISS)100 mg/kg,并于给药后1 h,24 h,48 h时分别取受损脊髓组织,利用测磷法检测Ca2+,Mg2+-ATP酶活性。结果:Res 100 mg/kg对Ca2+-ATP、Mg2+-ATP及Ca2+,Mg2+-ATP酶活性在SCI后的活性抑制有明显的改善作用(P<0.05或0.003),最大改善率分别在71％、180％和120％以上,基本以1 h时影响较大,但对Mg2+-ATP酶活性的最大影响在48 h处,MPSS对SCI后1h Ca2+、Mg2+-ATP酶活性影响最大。Res的作用与MPSS相当或更强。结论:Res通过调节Ca2+,Mg2+-ATP酶活性改善SCI后局部微环境,可能有效缓解SCI后继发性反应而产生保护作用。

Objective: To study the effects of resveratrol on Ca2 + , Mg2+ -ATPase activities after spinal cord trauma in rats. Methods: The weight-dropping method was used to produce the experimental spinal cord mjury(SCI) in adult rats. 500 mg/kg, 100 mg/kg resveratrol (Res and 100 mg/kg methylprednisolone (MPSS) were given intraperitoneally respectively, immediately after SCI. And then the effects of Res on Ca2+ ,Mg2+-ATPase activities were observed at 1h, 24 h,48 h after SCI and compared with MPSS. Results: Res could obviously augmem Ca2 + -ATPase, Mg2+-ATPase and Ca2+ ,Mg2+-ATPase activities almost all at 1 h after SCI in rats(P< 0.05 或 0.003) with the greatest improving rates over 71% , 180% , 120% , but at 48 h for Mg2+-ATPase. The effects of Res were the same as or greater than those of MPSS. Con elusion: Res may effectively protect the spinal cord through improving Ca2+ , Mg2+-ATPase system in SCI so as to inhibit the Ca2+ , Mg2+ ionic micro-environment change in the injured spinal cord. It suggested that Res may be having potent therapeutic effect on SCI.