点腺过路黄正丁醇提取部分对小鼠免疫反应的影响

The Effects of n-Butanol Fraction of Lysimachia hemsleyana on the Activities of Lymphocytes in Mice

目的:观察点腺过路黄正丁醇提取部分体外对小鼠腹腔巨噬细胞吞噬功能及脾淋巴细胞释放IL-2的影响。方法:用极性递增的溶剂顺次萃取出全草成分,研究其中的正丁醇提取部分对小鼠腹腔巨噬细胞吞噬功能及脾淋巴细胞释放IL-2的影响。结果:正丁醇提取部分(10-10～10-6g/ml)对脾淋巴细胞释放IL-2具有双向调节作用,并增强巨噬细胞的吞噬功能。结论:点腺过路黄可能具有免疫调节功能,对一些炎症的病理过程和自身免疫性疾病的治疗具有潜在的应用前景。

Objective: To study the effects of n-butanol fractoion from the whole plants ( Lysimachia hemsleyana) on the phagocytic activities of the peritoneal macrophage, production of interleukin-2. Methods; We extracted the components with ethaol, acetic ether, n-butanol and chloroform, then studied the n-butanol fraction on the phagocytic activities of the peritoneal macrophage, production of interleukin-2 (IL-2) from splenocytes induced by ConA in mice in vitro. Results; n-Butanol fraction of an ethanol extract of air-dried herbs bilaterally regulated the IL-2 production from splenocytes induced by ConA, and enhanced the phagocytic activites of activated mouse peritoneal macrophages. Conclusion: The results suggest that Lysimachia hemsleyana may has an immunoregulation effect and may be an potential agent in the treatment of the inflammatory pathological process and some autoimmune diseases.