Th1型/Th2型细胞因子对蜕膜细胞活性及其泌乳素分泌的影响

Effect of Th1-type/Th2-type cytolines on viability of decidual cells and secretion of PRL in vitro

目的 :研究Th1型 /Th2型细胞因子对人孕早期蜕膜细胞活性及泌乳素 (PRL)分泌的影响。方法 :蜕膜细胞活性采用MTT法进行检测 ,蜕膜组织分泌PRL的活性采用放免法 (RIA)进行分析测定。结果 :Th1型细胞因子IFNγ低浓度时 (1～ 10ng/ml)对蜕膜细胞活性及PRL分泌有促进作用 (P <0 0 5 ) ,高浓度时 (10 0～ 10 0 0ng/ml)则有抑制作用 (P <0 0 1)。而Th2型细胞因子IL 4在一定浓度范围内 (1～ 10ng/ml)可明显促进蜕膜的上述功能 (P <0 0 1)。结论 :Th1型

Objective:To study effect of Th1 type/Th2 type cytokines on viability of human decidual cells and secretion of PRL(prolactin)by decidual tissues in early pregnancy.Methods:The viability of decidual cells was detected by MTT.Radioimmunoassay(RIA) were applied to determine secretion of PRL by decidual tissues.Results:The low dose of Th1-type cytokines IFNγ(1～10ng/ml) can stimulate viability of decidual cells and secretion of PRL( P <0 05),but the high dose of that(100～1000ng/ml)can inhibit the above decidual functions( P <0 01).A ranged dose of Th2-type cytokines IL 4 (1～10ng/ml) can significantly enhance the above decidual functions( P <0 01).Conclusion:Th1-type/Th2-type cytokines play a important role of immune regulation in early pregnancy by influencing viability of decidual cells and secretion of PRL likely.