^(60)Coγ射线照射的新生牛血清对培养细胞的影响

Effect on Cell Culture by ^(60)Coγ-Irradiated Newborn Bovine Serum

为了获得质量合格的牛血清用于大规模细胞培养 ,以 3种不同剂量γ射线照射的新生牛血清加入细胞培养液 ,并用加热灭活的牛血清做对照培养CHO -C2 8细胞 ,观察两种灭活方法对除去牛血清中热原的效果及对细胞贴壁、生长增殖及分泌HBsAg的影响。结果表明 ,γ射线照射可除去牛血清中的热原质 ,在培养前期试验组牛血清对细胞贴壁和细胞生长增殖效果优于对照组。在培养中期 ,当γ射线的照射剂量为 2 0KGy时 ,细胞生长增殖和分泌HBsAg与对照组接近 ,两者无显著性差异 (P >0 .0 5 )。当照射剂量≥ 3 0KGy时 ,可抑制细胞分泌HBsAg ,与对照组相比有显著性差异 (P <0 .0 5 )。因而在细胞培养中采用γ射线照射牛血清以除去热原质是可行的 ,但照射剂量应≤ 2 0KGy。

To gain good quality newborn bovine serum on large scale cell culture. CHO C 28 cells were cultured with different preparation of medium in which newborn bovine serum were irradiated by three different dose of γ rays and the newborn bovine serum was inactivated by heating 56℃ 1hr as control .If pyrogen can be removed and adherence, proliferation and secretion of HBsAg of the cells were observed . The results showed that pyrogen can be removed in the serum by irradiation of γ rays .The test grope were better than the control grope on the cell adherence and proliferation in the prophase. The cell proliferation and secretion HBsAg in the test group were nearly same as in the control group (P>0.05)when irradiation dose ofγ rays was 20kGy.It can inhibit secretion HBsAg(P<0.05), when the irradiation dose was≥30kGy in the metaphase. Therefore the inactivated newborn bovine serum by γ irradiation is practical in this cell culture process and the irradiation dose should be ≤20kGy.