定向毁损杏仁核不同核群对大鼠行为的影响

The effect of different nucleus of amygdala lesion on behavior of SD rat

目的 研究毁损大鼠杏仁内、外侧核后行为的变化 ,探讨杏仁内、外侧核在非目的性行为、探究行为以及自我奖赏行为调节中的意义。方法 用立体定向技术对大鼠杏仁核的内、外侧核进行电流毁损 ,用空旷实验和糖水消耗实验测定毁损组和对照组大鼠自由活动量 (爬行格数 )、探究行为 (直例立次数 )以及自我奖赏行为指标的变化并进行分析。结果 外侧核毁损组空旷实验中直立次数为 3.2 5± 1.5 8,明显少于对照组 11.38± 5 .0 1,(P<0 .0 1) ,每日糖水消耗量分别为 4 5 .17± 10 .36和 6 3.6 6± 16 .35 ,两者相比有显著差异 (P <0 .0 5 ) ,但爬行格数与对照组相比无显著差异 (P <0 .0 5 ) ;内侧核毁损组直立次数为 6 .13± 4 .5 5 ,显著少于对照组 11.88± 5 .74 ,两者相比有显著差异 (P <0 .0 5 ) ,爬行格数和每日糖水消耗量与对照组相比无显著性差异 (P >0 .0 5 ) ;杏仁内、外侧核相比外侧毁损组直立次数显著低于内侧毁损组 (P <0 .0 5 )。结论 杏仁核参与动物对新奇环境的探究和自我奖赏行为 ,其中外侧核的作用尤其重要。对于非目的性的行为 ,杏仁核的内、外侧核群无明显影响。

Objective To study the behavior changes of rats after the lateral and median nucleus of amygdala were destructed and the significance of motivation?non-motivation and reward conduct regulated by the amygdala. Methods The lateral and median nucleus of amygdala were destructed by direct current using steroetactic technique. The crossing?rearing were measured by Open-Field test and sugar liquid consumption(SLC) test.Results In the lateral nucleus lesion group,the rearing and crossing times were 50.88±17.44 and 3.25±1.58 respectively, the SLC was 45.17±10.36g , contrasting with the lateral control group , rearing times decreased great ( P < 0.01) and the SLC abated sharply ( P <0.05) but there was no remarkable difference . In the median nucleus lesion group, the rearing times were 6.13±4.55, remarkably decreased contrasting with the control group ( P <0.05),Crossing and SLC between two groups had no significant difference. Conclusions Amygdala, especially the lateral nucleus,took part in researching behavior in novel environment and reward behavior, the median and lateral nucleus had no effect on non-motivation behavior.