摘要
Laminine(Ln) has been identified as an important marker of liver fibrosis or cirrhosis. The Ln immunosensor was formed with thioglycolic acid to produce a self-assembled monolayer on the gold electrode surface. The monoclonal Ln antibody was orderly immobilized on the modified gold surface covalently using EDC/NHS. An unlabeled antigen combined on the antibody could reduce the capacitance of the electrode, which was evaluated by the potentiostatic method. The measurement was performed in microseconds. Ln antigen could be determined in the range of 2-50 ng/mL. The quick response made the immunosensor a promising clinical screening method.
Laminine(Ln) has been identified as an important marker of liver fibrosis or cirrhosis. The Ln immunosensor was formed with thioglycolic acid to produce a self-assembled monolayer on the gold electrode surface. The monoclonal Ln antibody was orderly immobilized on the modified gold surface covalently using EDC/NHS. An unlabeled antigen combined on the antibody could reduce the capacitance of the electrode, which was evaluated by the potentiostatic method. The measurement was performed in microseconds. Ln antigen could be determined in the range of 2-50 ng/mL. The quick response made the immunosensor a promising clinical screening method.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2003年第1期46-48,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :39970 195
2 990 5 0 0 1和 2 0 0 0 5 0 0 2 )
长春应用化学研究所电化学国家重点实验室
上海市青年科技启明星计划资助
复旦大学三年行动规划"交叉项目资助计划"资助
