光动力学疗法新型光敏剂的光谱特性研究

Spectral Properties of New Photosensitizers for Photodynamic Diagnosis and Therapy

实验研究了用于光动力学诊断和治疗的新型光敏剂二磺基二邻苯二甲酰亚胺甲基酞菁锌(ZnPcS2 P2 )、癌光啉 (PsD 0 0 7)、血啉甲醚 (HMME)以及早期应用于临床的血卟啉衍生物 (HpD)分别在生理盐水和含 10 %人血清生理盐水中的光谱特性。结果表明 :除ZnPcS2 P2 的最大吸收峰位于 6 70nm之外 ,其余三种光敏剂在人血清环境中的最大吸收峰都位于 4 0 5nm处 ,但与生理盐水环境相比索瑞 (Soret)峰发生了 12nm的红移。在波长为 4 13和 5 14 5nm光源激发下 ,HMME ,HpD和PsD 0 0 7在人血清环境中的荧光发射峰都分别位于6 2 5和 6 90nm ,但 4 13nm光源的激发效率比 5 14 5nm光源高出 3倍左右 ,而且HMME的荧光激发效率最高 ,HpD次之 ,PsD 0 0 7最低。

The spectral properties of new photosensitizer ZnPcS 2P 2,PsD 007 and HMME, as well as traditional photosensitizer HpD have been studied by comparing their spectra in physiological saline and in physiological saline with 10 percent serum.Experimental results show that the maximum absorption peaks for PsD 007,HMME and HpD in the physiological saline with 10 percent serum appear at 400nm in the soret region, while at 670 nm for ZnPcS 2P 2. The fluorescence excitation spectra closely resemble the absorption spectra. When excited by the light at the wavelengths of 413 and 514 5 nm, the fluorescence emission peaks for PsD 007, HMME and HpD appear at 625 and 690 nm, respectively. The fluorescent excitation efficiency of the same photosensitizer with the same concentration excited by the light at the wavelength of 413 nm is about three fold higher than that at 514 5 nm. Furthermore, the fluorescent excitation efficiency is the highest for HMME,but is lower for HpD and lowest for PsD 007. These results are significant in the selection of photosensitizers for photodynamic diagnosis and therapy.