颈复康冲剂对异常应力环境兔颈椎间盘蛋白聚糖水平的影响

The Effect of Jingfukangchongji on the Changes of Proteoglycan of Cervical Intervertebral Disc under Long Term Abnormal Stress Condition

[目的 ]观察颈复康冲剂对异常应力环境下兔颈椎间盘蛋白聚糖 (PG)含量及组分变化的影响 ,探讨其防治颈椎病的可能机制。 [方法 ]将 32只家兔分为对照组、模型组、预防组与治疗组 ,后三组家兔颈椎处于屈曲 4 5°位的异常应力环境下 5小时 /次·天 ,预防组于造模开始及治疗组于造模 2个月后给予颈复康冲剂灌胃治疗 ,取 C5 - 6颈椎间盘生化测定PG及各组分的含量 ,比较各组间可能存在的差异。 [结果 ]对照组 PG总量和各组分含量均无显著变化 ;模型组、预防组和治疗组颈椎间盘 PG总量及各组分含量均呈明显下降趋势 (P<0 .0 5 ) ,实验 2个月时预防组 PG总量明显高于模型组 (P<0 .0 5 ) ,但各组分含量无显著差异 ,实验 3个月时模型组、预防组和治疗组 PG总量与各组分含量均无明显差别 (P>0 .0 5 )。 [结论 ]颈复康能够一定程度减少异常应力颈椎间盘 PG总量的丢失 。

To observe the effect of Chinese herbs( jingfukangchongji) on the changes of proteoglycan components of cervical intervertebral disc under long term abnormal stress condition .Thirty two Japanese white rabbits were randomly divided into control, model, prevention and treatment groups. All the rabbits in the latter three groups were kept at 45°flexion for 5 hours once daily. The prevention group were perfused with jingfukangchongji at the initial stage, and the treatment group was perfused two months later. After sacrificed, C5~6 intervertebral disc was chosen to biochemically determine PG and its components content. All the results were compared among the groups.In the control group, PG components showed no significant changes, and in the model, prevention and treatment group, a significant decrease of PG content was observed (P< 0 05). Prevention group had a higher PG content than that of the model group after two months (P<0 05), and after three months of the experiment, no significant differences were seen among the model, prevention and treatment groups (P>0 05). [Conclusion]Chinese herbs jingfukangchongji could slower the decrease of PG of cervical intervertebral disc, but could not change its components.