M_3R介导逼尿肌细胞收缩与信使分子IP_3关系的实验研究

RELATIONSHIP BETWEEN IP_3 AND DETRUSOR CELL CONTRACTION MEDIATIED BY M_3R

目的 :探讨信使分子IP3 在M3 R介导逼尿肌细胞收缩中的作用。方法 :MR非选择性的激动剂 (carba chol)、拮抗剂 (atropine)及M2 R拮抗剂 (methoctramine)、M3 R拮抗剂 (4-DAMP)刺激原代培养人逼尿肌细胞 ,通过 [3 H]掺入法 ,检测磷脂酰肌醇 (PI)的代谢产物 [3 H]-IP含量。结果 :[3 H]-IP的含量随carbachol刺激浓度的增加而增加 ;atropine和 4 -DAMP能显著抑制carbachol诱导的代谢反应 (P <0 .0 1) ,而methoc tramine不能显著抑制carbachol的作用 ;atropine与 4 -DAMP的作用相比无显著性差异 (P >0 .0 5 )。结论 :M3 R与PI分解产生的第二信使分子IP3 密切相关。

Objective:To study the relationship between IP 3 and detrusor cell contraction mediatied by M 3R. Methods:The -IP contents of human cultured detrusor cells were detected after stimulated by carbachol, atropine, methoctramine, 4-DAMP respectively.Results:The -IP contents increased with carbachol concentration. Both atropine and 4-DAMP signficantly inhibited the effect of carbachol on PI decomposition ( P < 0.01), but methoctramine didn't; The effect on PI decomposition between atropine and 4-DAMP had no significant difference ( P >0.05).Conclusion: M 3R is much related to IP 3.