一氧化氮合酶在肾脏缺血预处理中的表达

EXPRESSION OF NOS IN RENAL ISCHEMIC PRECODITIONING

目的 :检测一氧化氮合酶 (NOS :eNOS ,iNOS ,nNOS)在肾脏缺血预处理动物模型肾组织中的表达 ,探讨NOS在肾脏缺血预处理中的可能作用机制。方法 :将 36只小白兔随机分为四组即对照组、缺血预处理组(IP)、缺血再灌注组 (I/R)和缺血预处理后再灌注组 (IP +I/R)。分别检测各组肾组织中NOS的表达及组织学变化。结果 :在急性肾缺血 6 0min再灌注 6 0min时 ,eNOS阳性表达在IP组和IP +I/R组明显高于I/R组和对照组 (P <0 .0 1) ,iNOS和nNOS在组织肾中未见明显表达。组织学变化发现I/R组肾组织中肾细胞发生明显变性坏死 ,而IP组和对照组未见明显改变。结论 :肾脏缺血预处理在肾脏缺血再灌注中具有保护作用 ,而NOS参与该作用机制。

Objective:To determine the expression of NOS (eNOS, iNOS, nNOS) in renal ischemic preconditioning in an animal model, and to detect the possible function machanism of NOS in renal isehemic preconditioning.Methods:In a right-nephrectornized rat model, 36 animals were randomly put in four groups:the control group (sham), ischemic-preconditioning group (IP), ischemia reperfusion group (I/R), ischemia-reperfusion after ischemic-preconditioning group (IP+I/R). NOS expression in the kidney tissue was detected by the immuno-histochemical method,and histology assessed under the electromicroscope.Results:There was a higher eNOS expression in the IP-only group and in the IP+I/R group than in the control and I/R group ( P <0.05). The expression of iNOS and nNOS in each group was not detected. The histological assessment showed less evidence of cellular damage in the IP and IP+I/R than in the I/R group,and serum creatinine level was higher in I/R than in other groups.Conclusion:Ischemic-preconditioning has a protective effect on renal structure and function, and NOS is one of the important factors for its metchemism.